Aim: One of the most important risk factors proposed for gastric cancer is Helicobacter pylori; however, the correlation between polymorphism of TNF-α and TNFβ with Helicobacter infection has never been studied. The present research seeks to examine the correlation between polymorphism of these two genes in Helicobacter pylori infection and gastric cancer among those suffering from infection with this bacteria.
Material and Method: This is a case-control research seeking to study the polymorphism of TNF-α and TNF-β genes among those suffering from Helicobacter pylori infection and compare it to healthy people. Polymorphism genotype of TNF-α-308 and TNF-α+254 genes was studied in 31 healthy cases, 50 cases with H. pylori, and 23 cases with Helicobacter pylori and gastric cancer using ARMS & PCR-RFLP polymerase chain reaction method.
Results: According to the results obtained in this research, there is a significant correlation between TNF-308 A/A homozygote genotype and A allele in TNF-308 with H pylori. In other words, there is a correlation between TNF-308 A/A and the possibility of affliction with infection (P < 0.05). The correlation between TNF-308 G/A genotype and TNF-308 A/A genotype and TNF-308 An allele with affliction with cancer and H pylori was also significant (P < 0.05).
Discussion: TNF-308 A/A homozygote genotype has a significant correlation with gastric cancer and H pylori. As a matter of fact, TNF-308 A/A has increased the possibility of affliction with H pylori and gastric cancer. A significant correlation was also observed between TNF-308 G/A genotype and TNF-308 A/A and TNF-308 An allele with affliction with gastric cancer and H pylori.