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Abstract

Evaluation expression icaABCD biofilm formation genes in Staphylococcus aureus by Real-time PCR


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Authors
Pedram Ehterami, Mahboobeh Rajabpour, Sahar Honarmand Jahromy, Fatemeh Noorbakhsh

J Clin Anal Med 2017;8(Suppl 4):-
doi:10.4328/JCAM.5479

Abstract

Aim:Staphylococcus aureus  is one of the most significant pathogens that causes several nosocomial and community infections. Adhesion to surfaces and biofilm formation is considered main step in staphylococcal infection.  The aim of this study was to investigate the expression ica genes in clinical isolates of S.aureus.

Material and method: A total of 93 clinical S. aureus isolates was collected from hospitals in Tehran. Quantitative biofilm formation was determined by using Congo red agar (CRA). Out of 93 isolates, only 18 (19.35%) isolates positive biofilm formation were examined for expression the icaABCD genes by Real-time PCR method.

Results: The Congo red agar assay results showed that attachment abilities in 4 (4.3%) strains were strong and in 14 (15.05%) strains were moderate. All isolates were positive for icaABCD genes expression. The average gene expression for 18 strains was as follows for icaA (9.332), icaB (1.485), icaC (17.612) and icaD (3.390), respectively.

Conclusion: The data obtained suggest that clinical S. aureus isolated from the patients evaluated has a potential for biofilm formation. S. aureus clinical strains have different capacity to production biofilm. This may be caused by a different in the expression of biofilm genes and hetrogenicity in genetic origins.



Keywords : Staphylococcus aureus, Biofilm, icaABCD genes expression,

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How to Cite :
Pedram Ehterami, Mahboobeh Rajabpour, Sahar Honarmand Jahromy, Fatemeh Noorbakhsh, Evaluation expression icaABCD biofilm formation genes in Staphylococcus aureus by Real-time PCR , J Clin Anal Med 2017;8.(Suppl 4):-