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Abstract

Molecular Identification Of Types Tem, Shv And Ctx Extended-Spectrum-B-Lactamase Of Escherichia Coli


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Authors
Mohammad Taghi Akhi , Peyman Gholmohammadi , Reza Ghotaslu, Javid Sadeghi , Behrooz Naghili, Aydin Akhi, Somayeh Shiralizadeh

J Clin Anal Med 2017;8(Suppl 4):-
doi:10.4328/JCAM.5484

Abstract

Aim: Healthy ESBL carrier patients are the major challenge in control of infections produced by members of Enterobacteriaceae. The aims of the present study were to investigate the isolation of TEM, SHV, and CTX type ESβLs producing E. coli, Edwardsiella, and Erwinia spp. from feces of carriers.

Material and Methods: Two hundred fresh stool samples collected from non-hospitalized and hospitalized patients were cultured on MacConkey agar supplemented with 2 mg/L cefotaxime. After 24 hr. Incubation at 37C the E. coli, Erwinia and Edwardsiella spp were identified by routine biochemical tests. Combined tests were carried out to select ESBLs producing bacteria and susceptibility of isolates was determined by disc diffusion method. Multiplex-PCR was used to identify TEM, SHV and CTX type ESβLs producing isolates.

Results: Of the 34.5% bacteria resistant to cefotaxime, 81.63% and 55% ESBL producing organisms were recovered from inpatients and outpatients respectively. E. coli was the predominant ESBL-producing organism; One Erwinia and three Edwardsiella producing ESBL were detected. Overall, carbapenems including imipenem and meropenem and amikacin were the antibiotics most active against the ESBL-producing organisms. The overall prevalence of these ESBL genes was 73.92%, including the blaTEM and blaSHV genes alone in 27.45% and 5.88% respectively; blaCTX-M were not distinguished alone in any of the isolates

Conclusion: More than one ESBL was produced by most isolates carried by patients and Carbapenems, imipenem and meropenem continue to show good in vitro activity against the isolates. Patients can act as a source of ESBL producing bacteria in hospitals. 

 


Keywords : Healthy carrier, ESBL, E. coli, Erwinia, Edwardsiella,

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How to Cite :
Mohammad Taghi Akhi , Peyman Gholmohammadi , Reza Ghotaslu, Javid Sadeghi , Behrooz Naghili, Aydin Akhi, Somayeh Shiralizadeh, Molecular Identification Of Types Tem, Shv And Ctx Extended-Spectrum-B-Lactamase Of Escherichia Coli , J Clin Anal Med 2017;8.(Suppl 4):-