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Abstract

+45t>g single nucleotide polymorphism of adiponectin gene- is it a factor in childhood obesity?


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Authors
TUBA KASAP, Omer Ates, Samet Ozer, Ali Gul, Resul Yilmaz, Ergun Sonmezgoz, Osman Demir, Emel Ensari

J Clin Anal Med 2018;9(5):376-380
doi:10.4328/JCAM.5735

Abstract

Introduction: Childhood obesity is increasing in incidence and is strongly associated with obesity in adulthood. Several studies to explain the role of genetics in the pathogenesis of obesity were performed.

Materials and Methods: 268 obese and 185 healthy (control) children and adolescents aged 6-17 years were enrolled. Laboratory tests including fasting glucose, insulin levels and lipid profiles have been drawn only from the obese participants. The +45T>G SNP in adiponectin gene was analyzed from both group members by a polymerase chain reaction (PCR)-based restriction fragment length polymorphism (RFLP) method.

Results: The genotype frequencies of adiponectin gene for +45 locus in exon 2 were 77.9, 19.1 and 3% in obese subjects and 72.1, 23.5 and 4.4% in control group for TT, TG and GG respectively (p=0.357), with the allelic frequency of the G allele 12.5% in obese subjects and 16.1% in controls (p=0.129). When we compare different adiponectin genotypes, there wasn’t any significant difference in frequencies of TT genotype (wild type) and non-TT (TG+GG) genotypes between obese and control groups (p=0,162). Also comparing GG genotype and non-GG (TG+TT) genotypes revealed no significant difference between obese and control groups (p=0,439). Among obese subjects there were no significant relations between different genotypes and clinical characteristics such as presence of hypertriglyceridemia, low high density lipoprotein (HDL-C), hypertension and insulin resistance.

Conclusion: In conclusion, in the present study, in a well-described obese and control group of pediatric patients, we observed lack of association between the +45T>G SNP and childhood obesity and its traits.



Keywords : Childhood obesity, adiponectin, single nucleotide polymorphism,

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How to Cite :
TUBA KASAP, Omer Ates, Samet Ozer, Ali Gul, Resul Yilmaz, Ergun Sonmezgoz, Osman Demir, Emel Ensari, +45t>g single nucleotide polymorphism of adiponectin gene- is it a factor in childhood obesity? , J Clin Anal Med 2018;9.(5):376-380